ICTS:32885

Roles of the initiator tRNA in faithful translation and ribosome maturation in bacteria

(2025). Roles of the initiator tRNA in faithful translation and ribosome maturation in bacteria. SciVideos. https://scivideos.org/index.php/icts-tifr/32885

Roles of the initiator tRNA in faithful translation and ribosome maturation in bacteria. SciVideos, Oct. 02, 2025, https://scivideos.org/index.php/icts-tifr/32885 

          @misc{ scivideos_ICTS:32885,
            doi = {},
            url = {https://scivideos.org/index.php/icts-tifr/32885},
            author = {},
            keywords = {},
            language = {en},
            title = {Roles of the initiator tRNA in faithful translation and ribosome maturation in bacteria},
            publisher = {},
            year = {2025},
            month = {oct},
            note = {ICTS:32885 see, \url{https://scivideos.org/index.php/icts-tifr/32885}}
          }
Umesh Varshney
Talk numberICTS:32885
Source RepositoryICTS-TIFR

Abstract

 The process of translation initiation from an mRNA uses a special tRNA called initiator tRNA (tRNAfMet or i-tRNA). The i-tRNA is aminoacylated with methionine and then formylated with N10-formyl-tetrahydrofolate (N10-fTHF). Both methionine and N10-fTHF are produced via one-carbon metabolism providing a metabolic regulation of mRNA translation. The fidelity of translation initiation by i-tRNA is attributed to the structural features found in its acceptor-, and anticodon-, stems. The extent of i-tRNA formylation regulates its participation exclusively at the step of initiation or also at the step of elongation. Further, the pioneering round of initiation by i-tRNA triggers the final stages of ribosome maturation. In our recent work, use of an anticonvulsant drug, lamotrigine, has provided important insights into the mechanism of the role of IF2 in the process of ribosome maturation.