ICTS:29616

Video URL

Bridging DNA contacts allow E.coli Dps to condense the nucleoid

Bridging DNA contacts allow E.coli Dps to condense the nucleoid

(2024). Bridging DNA contacts allow E.coli Dps to condense the nucleoid. SciVideos. https://youtube.com/live/hirOAKbeQNY

Bridging DNA contacts allow E.coli Dps to condense the nucleoid. SciVideos, Sep. 16, 2024, https://youtube.com/live/hirOAKbeQNY 

          @misc{ scivideos_ICTS:29616,
            doi = {},
            url = {https://youtube.com/live/hirOAKbeQNY},
            author = {},
            keywords = {},
            language = {en},
            title = {Bridging DNA contacts allow E.coli Dps to condense the nucleoid},
            publisher = {},
            year = {2024},
            month = {sep},
            note = {ICTS:29616 see, \url{https://scivideos.org/index.php/icts-tifr/29616}}
          }
Sneha Shahu
Talk numberICTS:29616
Source RepositoryICTS-TIFR
Source EventInterdisciplinary aspects of chromatin organization and gene regulation
Collection

Abstract

The DNA-binding protein from starved cells (Dps) plays a crucial role in maintaining bacterial cell viability during periods of stress. Dps is a nucleoid-associated protein that interacts with DNA to create biomolecular condensates in live bacteria. Purified Dps protein can also rapidly form large complexes when combined with DNA in vitro. However, the mechanism that allows these complexes to nucleate on DNA remains unclear. Here, we examine how DNA topology influences the formation of Dps-DNA complexes. We find that DNA supercoils offer the most preferred template for the nucleation of condensed Dps structures. More generally, bridging contacts between different regions of DNA can facilitate the nucleation of condensed Dps structures. In contrast, Dps shows little affinity for stretched linear DNA before it is relaxed. Once DNA is condensed, Dps forms a stable complex that can form inter-strand contacts with nearby DNA, even without free Dps present in solution. Taken together, our re...