ICTS:29646

Video URL

Genome Compaction: Investigating Protein-DNA Condensation as a Mechanism

Genome Compaction: Investigating Protein-DNA Condensation as a Mechanism

(2024). Genome Compaction: Investigating Protein-DNA Condensation as a Mechanism. SciVideos. https://youtube.com/live/oSVbt4ZFxjA

Genome Compaction: Investigating Protein-DNA Condensation as a Mechanism. SciVideos, Sep. 17, 2024, https://youtube.com/live/oSVbt4ZFxjA 

          @misc{ scivideos_ICTS:29646,
            doi = {},
            url = {https://youtube.com/live/oSVbt4ZFxjA},
            author = {},
            keywords = {},
            language = {en},
            title = {Genome Compaction: Investigating Protein-DNA Condensation as a Mechanism},
            publisher = {},
            year = {2024},
            month = {sep},
            note = {ICTS:29646 see, \url{https://scivideos.org/icts-tifr/29646}}
          }
Sandeep Choubey
Talk numberICTS:29646
Source RepositoryICTS-TIFR
Source EventInterdisciplinary aspects of chromatin organization and gene regulation
Collection

Abstract

The organization of genome within the cell is essential for survival across all domains of life. The physical principles that govern genome organization remain elusive. Phase separation of protein and DNA has emerged as an attractive mechanism for reshaping and compacting the genome. In vitro studies have shed light on the biophysical principles of protein-DNA condensates driven by protein-protein and protein-DNA interactions. However, the role of DNA sequence and its impact on protein-DNA condensation remains elusive. Guided by experiments, we have developed a simple polymer-based model of protein-mediated DNA condensation that explicitly incorporates the influence of DNA sequence on protein binding. By employing coarse-grained Brownian dynamics simulations, we shed light on how DNA sequence affects the number, size and position of protein-DNA condensates. Comparing our simulation results with experimental data for the nucleoid-associated protein Lsr2 provides new insights into the me...