Compaction-mediated segregation of partly replicated bacterial chromosome
APA
(2024). Compaction-mediated segregation of partly replicated bacterial chromosome. SciVideos. https://youtu.be/IOaZL4UKa3s
MLA
Compaction-mediated segregation of partly replicated bacterial chromosome. SciVideos, Sep. 17, 2024, https://youtu.be/IOaZL4UKa3s
BibTex
@misc{ scivideos_ICTS:29644,
doi = {},
url = {https://youtu.be/IOaZL4UKa3s},
author = {},
keywords = {},
language = {en},
title = {Compaction-mediated segregation of partly replicated bacterial chromosome},
publisher = {},
year = {2024},
month = {sep},
note = {ICTS:29644 see, \url{https://scivideos.org/icts-tifr/29644}}
}
Abstract
Bacterial chromosome segregation, ensuring equal distribution of replicated DNA, is crucial for cell division. During fast growth, overlapping cycles of DNA replication and segregation require efficient segregation of the origin of replication (Ori), which is known to be orchestrated by the protein families SMC and ParAB. I will discuss our approach using data-driven physical modeling to study the roles of these proteins in Ori segregation. Developing a polymer model of the Bacillus subtilis genome based on Hi-C data, we analyzed chromosome structures in wild-type cells and mutants lacking SMC or ParAB. Wild-type chromosomes showed clear Ori segregation, while the mutants were segregation deficient. The model suggests the dual role of ParB proteins, loading SMCs near the Ori and interacting with ParA enriched at the cell poles, is crucial for Ori segregation. ParB-loaded SMCs compact individual Ori and introduce an effective inter-sister repulsion. While both the ParB-bound Ori tracks ...