ICTS:29629

Video URL

Enhancers on the Loose: Unveiling the determinants of susceptibility to disruption of chromatin str…

Enhancers on the Loose: Unveiling the determinants of susceptibility to disruption of chromatin structure.

(2024). Enhancers on the Loose: Unveiling the determinants of susceptibility to disruption of chromatin structure.. SciVideos. https://youtu.be/aPPbKzj5n6g

Enhancers on the Loose: Unveiling the determinants of susceptibility to disruption of chromatin structure.. SciVideos, Sep. 17, 2024, https://youtu.be/aPPbKzj5n6g 

          @misc{ scivideos_ICTS:29629,
            doi = {},
            url = {https://youtu.be/aPPbKzj5n6g},
            author = {},
            keywords = {},
            language = {en},
            title = {Enhancers on the Loose: Unveiling the determinants of susceptibility to disruption of chromatin structure.},
            publisher = {},
            year = {2024},
            month = {sep},
            note = {ICTS:29629 see, \url{https://scivideos.org/icts-tifr/29629}}
          }
Shreeta Chakraborty
Talk numberICTS:29629
Source RepositoryICTS-TIFR
Source EventInterdisciplinary aspects of chromatin organization and gene regulation
Collection

Abstract

CTCF-mediated chromatin loops play a crucial role in facilitating interactions between distal genomic regions. These loops have also been proposed to insulate enhancers from contacting with promoters in neighboring domains to prevent ectopic gene activation. However, the in vivo significance of this model has not been thoroughly tested. To test whether chromosome domains with higher density of developmental regulators are more susceptible to disruption of chromatin structure, we deleted a 25kb region containing four CTCF motifs at the boundary of a domain harboring the Fgf3, Fgf4, and Fgf15 loci. These genes with distinct spatiotemporal expression are critical for cell fate specification, patterning and organogenesis. Strikingly, heterozygous mutants showed perinatal lethality and encephalocele¬, a neural tube closure defect¬¬ caused by over-proliferation of neural tissue, abnormal cranial morphology, and skull bone hypoplasia. To confirm that these defects arise from loss of CTCF m...