Nanopore Sensing of DNA–Histone Complexes on Nucleosome Arrays
APA
(2024). Nanopore Sensing of DNA–Histone Complexes on Nucleosome Arrays. SciVideos. https://youtube.com/live/C78nCDOqNCM
MLA
Nanopore Sensing of DNA–Histone Complexes on Nucleosome Arrays. SciVideos, Sep. 15, 2024, https://youtube.com/live/C78nCDOqNCM
BibTex
@misc{ scivideos_ICTS:29623,
doi = {},
url = {https://youtube.com/live/C78nCDOqNCM},
author = {},
keywords = {},
language = {en},
title = {Nanopore Sensing of DNA{\^a}{\texteuro}{\textquotedblleft}Histone Complexes on Nucleosome Arrays},
publisher = {},
year = {2024},
month = {sep},
note = {ICTS:29623 see, \url{https://scivideos.org/icts-tifr/29623}}
}
Abstract
The location of nucleosomes in DNA and their structural stability are critical in regulating DNA compaction, site accessibility, and epigenetic gene regulation. Here, we combine the nanopore platform-based fast and label-free single-molecule detection technique with a voltage-dependent force rupture assay to detect distinct structures on nucleosomal arrays and then to induce breakdown of individual nucleosome complexes. Specifically, we demonstrate direct measurement of distinct nucleosome structures present on individual 12-mer arrays. A detailed event analysis showed that nucleosomes are present as a combination of complete and partial structures, during translocation through the pore. By comparing with the voltage-dependent translocation of the mononucleosomes, we find that the partial nucleosomes result from voltage-dependent structural disintegration of nucleosomes. High signal-to-noise detection of heterogeneous levels in translocation of 12-mer array molecules quantifies the het...