ICTS:29613

Video URL

Kinetic Proofreading in Transcriptional Regulation

Kinetic Proofreading in Transcriptional Regulation

(2024). Kinetic Proofreading in Transcriptional Regulation. SciVideos. https://youtube.com/live/sFsLB4iylFY

Kinetic Proofreading in Transcriptional Regulation. SciVideos, Sep. 16, 2024, https://youtube.com/live/sFsLB4iylFY 

          @misc{ scivideos_ICTS:29613,
            doi = {},
            url = {https://youtube.com/live/sFsLB4iylFY},
            author = {},
            keywords = {},
            language = {en},
            title = {Kinetic Proofreading in Transcriptional Regulation},
            publisher = {},
            year = {2024},
            month = {sep},
            note = {ICTS:29613 see, \url{https://scivideos.org/icts-tifr/29613}}
          }
Daniel R. Larson
Talk numberICTS:29613
Source RepositoryICTS-TIFR
Source EventInterdisciplinary aspects of chromatin organization and gene regulation
Collection

Abstract

The MYC oncogene has been studied for decades, yet there is still intense debate over how this transcription factor controls gene expression. We engineered an optogenetic variant of MYC (Pi-MYC) and combined this tool with single-molecule RNA and protein imaging techniques to investigate the role of MYC in modulating transcriptional bursting and transcription factor binding dynamics in human cells. We find that the mechanism by which MYC exerts global effects on the active period of genes is by altering the binding dynamics of transcription factors involved in RNA Polymerase II complex assembly and productive elongation. These studies expose fundamental questions about transcription regulation. Namely, how do transient interactions (~ seconds) between transcription factors and promoters lead to specific responses? We propose an extension of kinetic proofreading to explain transcriptional regulation in eukaryotes. This model suggests active, ATP-consuming processes drive transcription f...