ICTS:29611

Video URL

Chromatin organization and behavior during the cell cycle revealed by single-nucleosome imaging/tac…

Chromatin organization and behavior during the cell cycle revealed by single-nucleosome imaging/tacking

(2024). Chromatin organization and behavior during the cell cycle revealed by single-nucleosome imaging/tacking. SciVideos. https://youtube.com/live/E_JZ6F0i-1M

Chromatin organization and behavior during the cell cycle revealed by single-nucleosome imaging/tacking. SciVideos, Sep. 16, 2024, https://youtube.com/live/E_JZ6F0i-1M 

          @misc{ scivideos_ICTS:29611,
            doi = {},
            url = {https://youtube.com/live/E_JZ6F0i-1M},
            author = {},
            keywords = {},
            language = {en},
            title = {Chromatin organization and behavior during the cell cycle revealed by single-nucleosome imaging/tacking},
            publisher = {},
            year = {2024},
            month = {sep},
            note = {ICTS:29611 see, \url{https://scivideos.org/icts-tifr/29611}}
          }
Kazuhiro Maeshima
Talk numberICTS:29611
Source RepositoryICTS-TIFR
Source EventInterdisciplinary aspects of chromatin organization and gene regulation
Collection

Abstract

In higher eukaryotic cells, strings of nucleosomes, where long genomic DNA is wrapped around core histones, are irregularly folded into numerous condensed chromatin domains (1,2). Inside these domains, nucleosomes fluctuate and locally behave like a liquid (2,3). While nucleosome behavior is assumed to be highly related to genome functions, it remains unclear how this behavior changes during the cell cycle. During interphase, the nucleus enlarges and genomic DNA doubles. Previous reports have shown that chromatin movements vary during interphase on a minute or longer time-scale. However, using single-nucleosome imaging and tracking (4), we reveal that local nucleosome motion on a second time-scale remains steady throughout the G1, S, and G2 phases in live human cells (4). Combined with Brownian dynamics modeling, our results suggest that this steady-state nucleosome motion is mainly driven by thermal fluctuations. We propose that this observed steady-state nucleosome motion allows cell...